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KMID : 1101320090410020076
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Korean Journal of Clinical Laboratory Science
2009 Volume.41 No. 2 p.76 ~ p.82
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Forensic Evaluation of Prostate-Specific-Antigen (PSA) Rapid Test Kit for Identification of Human Semen
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Lim Chae-Won
Lee Jong-Hoon
Kim Hyung-Lak
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Abstract
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It would be one of the most important tests that determination of semen in crime scene as a matter of significant evidences. Recently, it has been developed for the identification of semen in forensic specimens which was used simply, easily and reproductively. In this study, Prostate-Specific-Antigen (PSA) Rapid Test kit was evaluated for the forensic identification of semen and compared with one step semen inspection forensic rapid test kit. The sensitivity and specificity of the rapid PSA kit were examined in addition to the stability of PSA. The positive band of rapid PSA kit shown even with 1,000,000-fold diluted semen, which was at least 100 timed higher than qualitative one step semen inspection forensic rapid test kit. PSA was detected in urine from normal male adult, however, it was not detected in urine from young boys and female body fluids. It was shown that PSA was very stable to resist boiling for 20 minutes and the effect of bacteria. In crime scene investigation, rapid PSA kit is expected to help to identify semen easily in the evidences.
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KEYWORD
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Semen, Prostate-Specific-Antigen (PSA), Sensitivity, Specificity, Stability
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